Keep up-to-date on the latest scripts and plugins at the FlowJo exchange. Are you getting the most from your analysis? Learn more at FJU Maximize what we can do for you with classes from FlowJo University.Plugins extend the functionality and power of FlowJo, which may be developed by 3rd-party developers or by our team. Activation of CD4+ and CD8+ T cells post infection. PBMC samples taken at W2-4-(A and B) or W23-PI (C and D) were stained with a mix of surface mAbs as described in Methods and S1 Table.
New Features: Fix issues in Mac OS 10.15 (Catalina) Update to use Java version 9; Improved support for JO workspace reading; Performance enhancing features. Java 8: FlowJo now uses Java 8, which brings a number of security and reliability improvements. This capability is now is distributed with the FlowJo install on both Windows and Mac. Mac OS Sierra (10.12): This OS is now fully supported.
New updates include:
- AutoSpill compensation: AutoSpill is a new algorithm for calculating spillover and producing a compensation matrix developed and incorporated into FlowJo v10.7 in collaboration with researchers from the Vlaams Instituut voor Biotechnologie in Belgium and the the Babraham Institute in Cambridge, UK.
- Our exclusive AutoSpread algorithm provides a Spillover Spreading Matrix (SSM) by calculating spillover spreading coefficients with linear models, removing the need for well-defined positive and negative populations in single-color controls.
'Together, AutoSpill and AutoSpread remove limiting constraints of traditional compensation methods, easing the preparation of compensation controls in high-parameter flow cytometry, making errors less likely, and facilitating the practical implementation of ultra high-parameter flow cytometry.' —Roca, et al
Read the full article
AutoSpill: a method for calculating spillover coefficients in high-parameter flow cytometry.
Authors: Carlos P. Roca, Oliver T. Burton, Teresa Prezzemolo, Carly E. Whyte, Richard Halpert, Łukasz Kreft, James Collier, Alexander Botzki, Josef Spidlen, Stéphanie Humblet-Baron, Adrian Liston. - Proliferation and Cell Cycle models have been updated to better fit data with missing peaks.
- The t-SNE derived parameters now remain connected to workspace files after saving. Note: In case derivative files are ever dissociated from a workspace, drag and drop the CSV back onto the data file or right click and choose “Import Parameters from CSV/CLR”.
- Figures added to the Layout Editor will no longer lose parameter annotations without user deselecting those features manually or setting them hidden in the preferences.
- Total Spread Matrix (TSM) is now available for matrices created in FlowJo. TSM is the Spillover Spreading Matrix (SSM) without normalization to the florescent intensity of the probe.
For more details on features in this release, and a comprehensive list of known issues visit the exhaustive release notes.
FlowJo macOS users: please read this update on Big Sur
Minimum requirements and previous versions
Before using your dongle, please read the following important information.
In most cases, you will find what you need here.
Important Information
A dongle is a small security device that plugs into your computer. It acts as a mobile serial number for software on any number of computers.
- Install or Download FlowJo:
- Do Not Rename the Dongle
The dongle should appear as “FJTHUMB” on Macintosh.
- Do Not Save Files to the Dongle
(.fcs, .wsp, .jo, .mp3, .jpg)
For additional dongle support, please email us at flowjooffice@bd.com or call 1-541-201-0022
Locating Your Dongle ID
Dongle ID Mac
1. Open Mac apple icon
2. Open ‘About This Mac’
3. Click on ‘System Report’
4. Under ‘Hardware’, click on USB
5. Under USB HighSpeed Bus, click on USB Disk
6. Look for ‘Serial Number’. This is the dongle ID
Mac Lion
1. Open Mac Apple Icon
2. Open “About this Mac”
2. Click “more info”
3. Click on the Storage tab, then click on Disk Utility
4. Click on “USB”
5. Then click “Serial ID”
Vista
1. Double-click the icon in the lower-right task bar that says “safely remove hardware”
2. Click on “Properties”, then ”Details”
3. Select “Device Instance Path” from the drop-down list
4. The dongle ID will appear as the last part of a line, everything after the slash mark (“”)
Windows 7
1. Navigate to Computer, then click on the dongle drive
2. Right-click for Properties, then click “Hardware”
3. Select flash drive, then “Properties”
4. Choose “Details”, then “Choose Device Instance Path”
5. The serial ID is everything after the last slash mark (“”)
Windows 8
1. Click “Desktop View”, then “file Explorer”
2. Right-click for Properties, then click “Hardware”
3. Select flash drive, then “Properties”
4. Choose “Details”, then “Choose Device Instance Path”
5. The serial ID is everything after the last slash mark (“”)
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Windows XP
1. Right-click on My Computer, then click “Properties”
2. Click the “Hardware” tab, then “Device Manager”
3. Under Disk Drive, find the find the USB drive and double-click on it
4. Click on the “Details” tab
5. Make sure that “Device Instance” is chosen.
6. The Serial ID for the dongle will appear after the slash mark (“”)
Dongle Troubleshooting Help
In the unfortunate event that your dongle stops working, please do the following:
- Is your dongle purple?Purple dongles are no longer supported and must be replaced. Please contact our office staff atflowjooffice@bd.comor call 1-541-201-0022 to have your purple dongle replaced.
- Update your version of FlowJo. (PC users, please make sure the proper version of Java is running)
- Safely eject the dongle, restart the computer, plug the dongle back to mount as a drive and restart FlowJo.
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Upgrade Information
Why Upgrade to the Newest Version?
FlowJo is a leader in state-of-the-art flow cytometry analysis software and version 10 features a new, more intuitive interface throughout. Our patented, hierarchical display, quick drag-and-drop cluster manipulation and flexible batch processing have all been optimized to offer the user a more fluid, interactive experience. To see all of the new features in FlowJo v10, visitdocs.flowjo.com.
Contact us
USA: 800-366-6045
International: +1 541-201-0022
or flowjooffice@bd.com for more information.
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FlowJo Dongle Replacement
- If a FlowJo dongle is defective due to its manufacturing, FlowJo will replace it without cost, and the faulty dongle must be returned to FlowJo, LLC.
- If a FlowJo dongle breaks due to rough handling, such as twisting it in the computer, dropping a laptop, hitting it with a vacuum cleaner or foot, a new dongle will be sold to the researcher for a replacement fee plus shipping. The broken dongle must be returned to FlowJo, LLC.
- If a FlowJo Dongle malfunctions, it is at the discretion of our dongle support team to replace or charge a replacement fee. If the dongle is 3+ years old, there will most likely be a replacement fee, because the dongle probably has been used extensively.
- If a dongle is missing or misplaced, the lab will need to purchase a new one at the regular rates.*
- If a dongle is stolen and there is an official police report documenting the theft, a new dongle will be sold to the researcher for a replacement fee plus shipping. *
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*We like to know which dongle is missing so that we can arrange to get it returned to the lab if the missing dongle is found.